Search results for "REACTIONS DE DEFENSE"
showing 2 items of 2 documents
The Plant Defense Elicitor Cryptogein Stimulates Clathrin-Mediated Endocytosis Correlated with Reactive Oxygen Species Production in Bright Yellow-2 …
2008
Abstract The plant defense elicitor cryptogein triggers well-known biochemical events of early signal transduction at the plasma membrane of tobacco (Nicotiana tabacum) cells, but microscopic observations of cell responses related to these early events were lacking. We determined that internalization of the lipophilic dye FM4-64, which is a marker of endocytosis, is stimulated a few minutes after addition of cryptogein to tobacco Bright Yellow-2 (BY-2) cells. This stimulation is specific to the signal transduction pathway elicited by cryptogein because a lipid transfer protein, which binds to the same receptor as cryptogein but without triggering signaling, does not increase endocytosis. To…
Rôle de la dynamique de la membrane plasmique dans la mise en place des réactions de défense chez le tabac
2008
The binding of cryptogein, a plant defense elicitor secreted by an oomycete, to an unknown receptor triggers well-known biochemical events of early signal transduction at the plasma membrane of tobacco cells. One of these early events is the transient production of Reactive Oxygen Species (ROS) by an NADPH oxidase, NtrbohD. We were thus interested to analyze the dynamic of the plasma membrane during these early stages. We determined that internalization of the endocytotic marker FM4-64 is stimulated a few minutes after addition of cryptogein to tobacco BY-2 cells. This stimulation is specific to the signal transduction pathway elicited by cryptogein since a lipid transfer protein, which bin…